Regulatory

Part:BBa_K1343018:Design

Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon   Group: iGEM14_Technion-Israel   (2014-10-06)

Ptet->tetR->T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
    Illegal XhoI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

BBa_R0040:TetR repressible promoter, BBa_B0030: Strong RBS, BBa_K145001:T7_polymerase (BL21DE3), BBa_B0010:T1 (terminator) from E. coli.

References